中圖分類號(hào) R284 文獻(xiàn)標(biāo)志碼 A 文章編號(hào) 1001-0408（2018）18-2515-05
　　DOI 10.6039/j.issn.1001-0408.2018.18.16
　　摘 要 目的：建立同時(shí)測(cè)定大花紅景天、狹葉紅景天和長(zhǎng)鞭紅景天藥材中沒(méi)食子酸、紅景天苷、酪醇、咖啡酸、對(duì)香豆酸含量的方法，并比較3種紅景天藥材中5種化學(xué)成分的含量差異。方法：采用高效液相色譜法測(cè)定含量，色譜柱為WondaSil C18，流動(dòng)相為乙腈-0.3%磷酸溶液（梯度洗脫），檢測(cè)波長(zhǎng)為275 nm，柱溫為25 ℃，流速為1.0 mL/min，進(jìn)樣量為10 μL；采用GraphPad Prism 5.0軟件對(duì)含量測(cè)定結(jié)果進(jìn)行單因素方差分析和Tukey’s多重比較。結(jié)果：沒(méi)食子酸、紅景天苷、酪醇、咖啡酸、對(duì)香豆酸的檢測(cè)進(jìn)樣量線性范圍分別為0.020 48～0.614 4 ?g（r=0.999 7）、0.118～3.54 ?g（r=0.999 5）、0.010 84～0.325 2 ?g（r=0.999 7）、0.008 48～0.254 4 ?g（r=0.999 5）、0.004 1～0.123 ?g（r=0.999 5）；精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD均小于2%；加樣回收率分別為99.62%～104.92%（RSD=1.68%，n=9）、96.55%～100.69%（RSD=1.67%，n=9）、98.91%～103.39%（RSD=1.48%，n=9）、100.93%～104.98%（RSD=1.37%，n=9）、97.71%～103.89%（RSD=1.99%，n=9）。大花紅景天中沒(méi)食子酸、紅景天苷、酪醇和咖啡酸含量均顯著高于長(zhǎng)鞭紅景天，大花紅景天中的紅景天苷、咖啡酸含量均顯著高于狹葉紅景天，差異均有統(tǒng)計(jì)學(xué)意義（P<0.05，P<0.01或P<0.001）。結(jié)論：該方法簡(jiǎn)便可行、結(jié)果準(zhǔn)確，可用于同時(shí)測(cè)定大花紅景天、狹葉紅景天和長(zhǎng)鞭紅景天藥材中沒(méi)食子酸、紅景天苷、酪醇、咖啡酸、對(duì)香豆酸的含量。3種常見(jiàn)紅景天藥材中的化學(xué)成分含量存在較大差異，大花紅景天中紅景天苷的含量最高。
　　關(guān)鍵詞 高效液相色譜法；單因素方差分析；Tukey’s多重比較；紅景天；沒(méi)食子酸；紅景天苷；酪醇；咖啡酸；對(duì)香豆酸；含量
　　ABSTRACT OBJECTIVE： To establish a method for simultaneous determination of gallic acid， salidroside， tyrosol， caffeic acid and p-coumaric acid in Rhodiola crenulata， R. kirilowii and R. fastigiata， and to compare the content difference of 5 chemical components in 3 kinds of R. rosea. METHODS： HPLC method was adopted. The separation was performed on WondaSil C18 column with mobile phase consisted of acetonitrile-0.3% phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 275 nm， and the column temperature was 25 ℃. Sample size was 10 μL. GraphPad Prism 5.0 software was used for single factor variance analysis and Tukey’s multiple comparison of content determination results. RESULTS： The linear ranges of gallic acid， salidroside， tyrosol， caffeic acid and p-coumaric acid were 0.020 48-0.614 4 ?g（r=0.999 7）， 0.118-3.54 ?g（r=0.999 5）， 0.010 84-0.325 2 ?g（r=0.999 7）， 0.008 48-0.254 4 ?g（r=0.999 5） and 0.004 1-0.123 ?g（r=0.999 5）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2%. The recoveries were 99.62%-104.92%（RSD=1.68%，n=9）， 96.55%-100.69%（RSD=1.67%，n=9）， 98.91%-103.39%（RSD=1.48%，n=9）， 100.93%-104.98%（RSD=1.37%，n=9）， 97.71%-103.89%（RSD=1.99%，n=9）， respectively. The contents of gallic acid， salidroside， tyrosol and caffeic acid in R. crenulata were higher than R. fastigiata； the contents of salidroside and caffeic acid in R. crenulata were significantly higher than R. kirilowii， with statistical significance （P<0.05，P<0.01 or P<0.001）. CONCLUSIONS： The method is simple， feasible and accurate， and can be used for simultaneous determination of gallic acid， salidroside， tyrosol， caffeic acid and p-coumaric acid in R. crenulata， R. kirilowii and R. fastigiata. There was great difference in the contents of chemical components in 3 kinds of common R. rosea， the content of salidroside is the highest in R. crenulata.

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