[摘要] 建立山茱萸藥材中環(huán)烯醚萜苷類及三萜酸類化學成分的含量測定方法，為山茱萸藥材的質量標準提升提供技術支撐。采用HPLC-UV法，以乙腈-0.1%甲酸水溶液為流動相梯度洗脫，檢測波長為240 nm，建立同時測定莫諾苷、馬錢苷、當藥苷的含量測定方法;采用HPLC-ELSD法，以甲醇-0.5%乙酸銨（87∶13）為流動相，建立同時測定齊墩果酸、熊果酸的含量測定方法。結果顯示在上述色譜條件下，莫諾苷、馬錢苷、當藥苷分別在質量濃度5.335～213.4 mg·L^-1（r = 0.999 9），5.515～220.6 mg·L^-1 （r=1.000 0），1.992～79.68 mg·L^-1（r=1.000 0）線性關系良好，平均加樣回收率均在98.49%～99.28%，RSD<2%;齊墩果酸、熊果酸分別在質量濃度7.74～154.8 mg·L^-1（r=0.996 4），10.82～216.4 mg·L-1 （r=0.999 6）線性關系良好，平均加樣回收率均在98.11%～99.27%，RSD <3%。表明所建立的方法簡單快速，準確可靠，重復性好，可用于客觀評價山茱萸藥材的品質及其質量控制，為完善山茱萸藥材現(xiàn)行質量標準提供了實驗數(shù)據(jù)，對于保證其質量和臨床療效具有重要意義。
　　[關鍵詞] 山茱萸;環(huán)烯醚萜苷;三萜酸;藥材質量標準制定
　　[收稿日期] 2015-04-12
　　[基金項目] 國家自然科學基金項目（81473538）;教育部霍英東教育基金會高等院校青年教師基金項目（141040）;江蘇高校優(yōu)勢學科建設工程資助項目（ysxk-2014）
　　[通信作者] *段金廒，教授，博士研究生導師，Tel： （025）85811116，E-mail： dja@njucm.edu.cn
　　[作者簡介] 郭盛，博士，主要從事中藥資源化學研究及教學工作，Tel：（025）85811916，E-mail：guosheng@njucm.edu.cn
　　Establishment of quality standard for Corni Fructus based on
　　the multiple bioactive constituents
　　GUO Sheng，  DUAN Jin-ao*，  ZHU Shao-qing，  QIAN Da-wei
　　（Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， and National and
　　Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative
　　Medicine， Nanjing University of Chinese Medicine， Nanjing 210023， China）
　　[Abstract] This study is to establish the methods for determination of iridoid glycosides and triterpenic acids in Corni Fructus and provide technical support for the quality control of Corni Fructus.Morroniside， loganin and sweroside were determined by HPLC-UV method with acetonitrile and 0.1% formic acid as the mobile phase， and the detective wavelength was set at 240 nm. Oleanolic acid and ursolic acid were determined by HPLC-ELSD method with methanol-0.5% ammonium acetate （87∶13） as the mobile phase. The results showed that  the linear ranges of morroniside， loganin and sweroside were 5.335-213.4 mg·L^-1 （r=0.999 9）， 5.515-220.6 mg·L^-1（r=1.000 0）， 1.992-79.68 mg·L^-1（r=1.000 0）， respectively. The average recoveries of the above three iridoid glycosides were 98.49%-99.28% with RSDs of recoveries being less than 2%.The linear ranges of oleanolic acid and ursolic acid were 7.74-154.8 mg·L-1 （r=0.996 4），10.82-216.4 mg·L-1 （r=0.999 6）， respectively. The average recoveries of the above two triterpenic acids were 98.11%-99.27% with RSDs of recoveries being less than 3%. The method established in this research is simple， rapid and reliable， and can be used for quality control of Corni Fructus. Furthermore， the research provided experimental data for the improvement of present quality standard of Corni Fructus， which has important significance to guarantee its quality and clinical curative effect.

相關熱詞搜索：山茱萸 藥材 質量標準 功效 成分